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柄袋沙蠋体壁和消化系统甲硫氨酸脑啡肽免疫组织化学定位
引用本文:黄雯雯,王宜艳,管晓娟,孙虎山.柄袋沙蠋体壁和消化系统甲硫氨酸脑啡肽免疫组织化学定位[J].四川动物,2011,30(4).
作者姓名:黄雯雯  王宜艳  管晓娟  孙虎山
作者单位:鲁东大学生命科学学院,山东烟台,264025
摘    要:采用免疫组织化学SABC法,对甲硫氨酸脑啡肽(M-ENK)在柄袋沙蠋体壁及其附属物鳃、疣足和消化系统各器官中的分布进行了研究.结果 表明,柄袋沙蠋体壁、鳃、疣足以及各消化器官的上皮细胞中均有不同程度的M-ENK阳性反应,且体壁、消化道的食管、肠、直肠中阳性反应多集中在上皮细胞的游离端;体壁肌肉中环肌层M-ENK阳性反应比纵肌层强;食道侧囊上皮细胞和结缔组织中也有M-ENK分布.M-ENK在柄袋沙蠋的体壁、鳃、疣足及消化系统各器官中均有分布,且分布密度不同,可能与各部位的功能有关.

关 键 词:柄袋沙蠋  甲硫氨酸脑啡肽  体壁  消化系统  免疫组织化学

Immunohistochemical Localization of Methionine-enkephalin in the Body Wall and Digestive System of Arenicola brasiliensis
HUANG Wen-wen,WANG Yi-yan,GUAN Xiao-juan,SUN Hu-shan.Immunohistochemical Localization of Methionine-enkephalin in the Body Wall and Digestive System of Arenicola brasiliensis[J].Sichuan Journal of Zoology,2011,30(4).
Authors:HUANG Wen-wen  WANG Yi-yan  GUAN Xiao-juan  SUN Hu-shan
Institution:HUANG Wen-wen,WANG Yi-yan,GUAN Xiao-juan,SUN Hu-shan(College of Life Science,Ludong University,Yantai,Shandong Province 264025,China)
Abstract:The methionine-enkephalin(M-ENK) in the body wall and its components gill,parapodia and digestive system of Arenicola brasiliensis was analyzed by the immunohistochemical strept avidin-biotin complex(SABC) staining.The results showed that the different degree of positive M-ENK immunoreaction was observed in the epithelium of body wall,gill,parapodia and each organ of the alimentary.Besides,the positive immunoreaction was centralized in the dissociative sides of the epithelium of the body wall,oesophagus,int...
Keywords:Arenicola brasiliensis  methionine-enkephalin  body wall  digestive system  immunohistochemistry  
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