麻黄素对仔鼠睾丸组织结构和Bax、EGF蛋白的影响

为探讨麻黄素对各发育期昆明小鼠(Mus musculus)仔鼠睾丸组织结构及Bax蛋白和表皮生长因子(epidermal growth factor,EGF)阳性表达的影响,选取雄性仔鼠采用递增剂量腹腔连续注射麻黄素,应用生物显微技术观察睾丸组织结构的变化,用免疫组织化学和体视学半定量方法检测Bax和EGF蛋白在睾丸组织中的表达.结果表明,麻黄素组仔鼠各发育阶段生精小管直径及上皮厚度明显小于对照组(P＜0.01或P＜0.05),初级精母细胞直径小于对照组(P＜0.01或P＜0.05),生精小管上皮各级生精细胞的发生较晚,且排列疏松而凌乱,细胞间空隙多,发育成熟期管腔内成熟精子较少；麻黄素组各发育期仔鼠睾丸中Bax和EGF蛋白的阳性表达与对照组相比显著增强(P＜0.01或P＜0.05).上述结果表明,麻黄素影响仔鼠睾丸组织的发育,麻黄素对各发育期仔鼠睾丸组织的损伤可能与Bax和EGF蛋白表达的增强有关.

Effects of Ephedrine on Histological Structure and Expression of Bax and EGF in Testis of Young Male Mice

To study the effects of ephedrine on histological structure of testis and on expression of Bax protein and epidermal growth factor (EGF)in the testis of the developing young mice (Mus musculus), ephedrine was administered by intraperitoneal injection at different doses. The testis structure was observed by microscopy and expression of Bax protein and EGF in the testis was measured by immunohistochemistry combined with stereological method at different developmental stages. Results indicated that the diameter and epithelium thickness of seminiferous tube significantly decreased in the experimental groups (P<0.01 or P<0.05). The diameter of primary spermatocyte was smaller than that of the control group (P<0.01 or P<0.05). Spermatogenic cells in seminiferous tube appeared late,and the cells loosely distributed,with more space between them, in the treatment group. At maturity there were less mature spermatozoa in the lumen of seminiferous tube. The expression of Bax protein and EGF in the testis of ephedrine-treated young mice significantly increased (P<0.01 or P<0.05). So the testis structure of young mice at different developmental stages were damaged to different degrees by ephedrine,which led to slow development of gonad. Therefore, ephedrine may affect spermatogenesis by altering status of primary spermatocytes and testis damage may be correlated with the expression intensity of Bax protein and EGF in young mouse testis.