干旱对小麦幼苗诱导蛋白表达与某些生理特性的初步探讨

试验以－１．２ＭＰａＰＥＧ６０００处理动小麦种子（ＴｒｉｔｉｃｕｍａｅｓｔｌｉｖｕｍＬ．）．ＳＤＳ－ＰＡＧＥ图谱分析表明，水分胁迫诱导幼芽及整株均产生４８．４ｋＤ、４１．５ｋＤ二个蛋白质亚基。在幼根中未出现以上二个蛋白亚基。胁迫４８ｈ后，根干重／芽干重比呈上升趋势，幼芽细胞膜楔对透性增大和相对含水量降幅度均大于幼根。

INITIAL STUDYS OF RELATIONSHIP BETWEEN THE APPEARANCE OF INDUCED-PROTEIN AND SOME PHYSIOLOGICAL CHARACTERS OF WHEAT SEEDLING IN WATER STRESS

Changes in protein profiles induced by water stress (-1. 2MPa PEG) were investigated in roots and shoot of germinating seeds of wheat.As revealed by SDS-PAGE,Two new proteins having molecular weights of 48. 4kD and 41.5kD, accumulated onlyin shoot and seedling.The two new proteins did not accumulated in roots. 48h aftertreatment with PEG at - 1. 2MPa, the degrees of the inhibition growth of shoots wasgreater than that the roots,and the degrees of the decrease of RWC and the increase ofmembrane permeability in shoots were higher than the roots. When water stressseedling were rewatered,shoot quickly resumed growth. The roots were partly died.Newroots developed later from the root sheath tissue. The degrees of the decrease of RWCand the increase of membrane permeability in roots were higher than the shoot when retreat.The results indicated that there is a correlation between dehydration survival andinduced protein accumulation.