Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study |
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| Authors: | da Fonseca Patrícia de Freitas Luis Alexandre Pedro Pinto Luis Felipe Ribeiro Pestana Cezar Rangel Bonato Pierina Sueli |
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| Institution: | Faculdade de Ciências Farmacaeuticas de Ribeir?o Preto, Universidade de S?o Paulo, S?o Paulo, Brazil. |
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| Abstract: | An enantioselective method using liquid-phase microextraction (LPME) followed by HPLC analysis was developed for the determination of oxybutynin (OXY) and its major metabolite N-desethyloxybutynin (DEO) in rat liver microsomal fraction. The LPME procedure was optimized using multifactorial experiments. Under the optimal extraction conditions, the mean recoveries were 61 and 55% for (R)-OXY and (S)-OXY, respectively, and 70 and 76% for (R)-DEO and (S)-DEO, respectively. The validated method was employed to an in vitro biotransformation study using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of OXY. |
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