Characterization of three capsular polysacharides produced by Burkholderia pseudomallei |
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Authors: | Kawahara K Dejsirilert S Ezaki T |
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Affiliation: | Department of Bacteriology, Kitasato Institute, 5-9-1 Shirokane Mmato-ku, Tokyo, Japan kawahara-k@kitasato.or.jp |
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Abstract: | Three kinds of capsular polysaccharide (CP) were found to be produced by Burkholderia pseudomallei. When the bacterium was grown with the medium without glycerol, CP-1a and CP-1b were produced. CP-1a was mainly 1.4-linked glucan and CP-1b was identified as a polymer composed of galactose and 3-deoxy-D-manno-octulosonic acid, whose chemical structure was recently reported by other laboratories. When the bacterium was grown with the medium containing 5" glycerol. CP-2 was synthesized. CP-2 contained galactose, rhamnose, mannose, glucose and a uronic acid in a ratio of approximately 3:1:0.3:1:1. Methylation analysis of the purified polysaccharides demonstrated that the two acidic polysaccharides. CP-1b and CP-2 shared no common structure, indicating that CP-2 was an acidic capsular polysaccharide whose chemical characters were not reported previously. |
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Keywords: | Burkholderia pseudomallei Melioidosis Capsular polysaccharide |
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