Morphology,Physiology, and Virulence of <Emphasis Type="Italic">Bipolaris sorokiniana</Emphasis> Isolates |
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Authors: | A Poloni I S Pessi A P G Frazzon S T Van Der Sand |
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Institution: | (1) Laboratório de Micologia Ambiental, Departamento de Microbiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, CEP 90050-170, Porto Alegre, RS, Brazil |
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Abstract: | Bipolaris sorokiniana is a phytopathogenic fungus that causes diseases in cereal crops. The high morphological, physiological, and genetic variability
makes the control of this fungus a difficult task. The aim of this work was to study the virulence, morphological, and physiological
variability of B. sorokiniana isolates. For this, 35 B. sorokiniana isolates from different geographic regions in Brazil and other countries were used. The isolates were evaluated for their
morphological variability, considering mycelium color, sector formation, and growth rate. Based on these morphological characteristics,
the isolates were grouped in five different morphological groups. Extracellular enzymes activity in solid medium, virulence
in wheat seeds and seedlings, and analysis of total proteins by SDS-PAGE were evaluated for all isolates. Variations among
the isolates were found for enzymatic activity, and esterase was the enzyme that showed the highest activity indices. The
results obtained from infection of seeds and seedlings showed that isolates from the same geographical region and morphological
group had different degrees of virulence. The total protein profile shown by the isolates varied in the number of bands and
intensity, where some of them may be used to characterize the specie. |
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