Oxidation of biotin during oligonucleotide synthesis |
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| Authors: | Upadhya Krishna Khattak Iqbal K Mullah Bashar |
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| Affiliation: | Applied Biosystems, 8a50 Lincoln Centre Drive, Foster City, CA 94404, USA. upadhykg@appliedbiosystems.com |
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| Abstract: | A new "polystyrene biotin support" has been synthesized for the solid support synthesis of the 3'-biotinylated oligonucleotides. Several oligos were synthesized and were analyzed by the HPLC and Mass Spec. Oligo analysis revealed that the biotin gets oxidized to "biotin sulfoxide" during the synthesis. |
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