A simple and eficient method for high fidelity PCR cloning using antibody-neutralizing technology |
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| Authors: | Kitabayashi Masao Nishiya Yoshiaki Esaka Muneharu |
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| Institution: | Graduate School of Biosphere Sciences, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan. |
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| Abstract: | We introduce the TA cloning antibody method for the high-fidelity PCR product amplified by family B DNA polymerase without purification. This method uses antibodies and Thermus aquaticus (Taq) DNA polymerase. The antibodies can inhibit only the activity of family B DNA polymerase, and Taq can co-work for A-tailing. This method has nearly cloning efficiency to that of the PCR product of Taq. |
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