Interaction of anthracycline antibiotics with actin and heavy meromyosin. |
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Authors: | A Someya T Akiyama M Misumi N Tanaka |
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Affiliation: | Institute of Applied Microbiology, University of Tokyo, Tokyo Japan |
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Abstract: | For the purpose of elucidating the biochemical mechanism of anthracycline cardiomyopathy, the interaction with actin and heavy meromyosin (HMM) was studied. HMM and acto-HMM Mg2+-ATPase reactions were inhibited by daunorubicin and adriamycin; but not significantly by aclacinomycin A. The three antibiotics induced G-actin polymerization. Difference absorption spectra showed a direct interaction of adriamycin or aclacinomycin A with actin or HMM. Equilibrium dialysis and spectrofluorometric studies indicated that actin monomer possesses one binding site for adriamycin or aclacinomycin A with the same order of association constants (1.4 – 7.2 × 104 M?1). Adriamycin exhibited significantly higher affinity for HMM than aclacinomycin A. |
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