Detection of Bcl-2 family member Bcl-G in mouse tissues using new monoclonal antibodies |
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| Authors: | M Giam J D Mintern G J P Rautureau M G Hinds A Strasser P Bouillet |
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| Institution: | 1The Walter and Eliza Hall Institute of Medical Research, Molecular Genetics of Cancer Division, Melbourne, Australia;2Department of Medical Biology, The University of Melbourne, Melbourne, Australia |
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| Abstract: | Bcl-G is an evolutionarily conserved member of the Bcl-2 family of proteins that has been implicated in regulating apoptosis and cancer. We have generated monoclonal antibodies that specifically recognise mouse Bcl-G and have used these reagents to analyse its tissue distribution and subcellular localisation using western blotting, immunohistochemistry and immunofluorescence. We found that Bcl-G predominantly resides in the cytoplasm and is present in a wide range of mouse tissues, including the spleen, thymus, lung, intestine and testis. Immunohistochemical analyses revealed that Bcl-G is expressed highly in mature spermatids in the testis, CD8+ conventional dendritic cells (DCs) in hematopoietic tissues and diverse epithelial cell types, including those lining the gastrointestinal and respiratory tracts. The Bcl-G monoclonal antibodies represent new tools for studying this protein, using a variety of techniques, including immunoprecipitation and flow cytometry. |
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| Keywords: | apoptosis Bcl-G Bcl-2 family |
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