Associative Cyanobacteria Isolated from the Roots of Epiphytic Orchids |
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Authors: | Tsavkelova E A Lobakova E S Kolomeitseva G L Cherdyntseva T A Netrusov A I |
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Institution: | (1) Department of Microbiology, Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia;(2) Department of Cell Physiology and Immunology, Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia;(3) Central Botanical Garden, Russian Academy of Sciences, Moscow, 127276, Russia |
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Abstract: | Associative cyanobacteria were isolated from the rhizoplane and velamen of the aerial roots of the epiphytic orchids Acampe papillosa, Phalaenopsis amabilis, and Dendrobium moschatum and from the substrate roots of A. papillosa and D. moschatum. Cyanobacteria were isolated on complete and nitrogen-free variants of BG-11 medium. On all media and in all samples, cyanobacteria of the genus Nostoc predominated. Nostoc, Anabaena, and Calothrixwere isolated from the surface of the A. papillosa aerial roots, whereas the isolates from the substrate roots were Nostoc, Oscillatoria,and representatives of the LPP group (Lyngbia, Phormidium, and Plectonema, incapable of nitrogen fixation). On the D. moschatum substrate roots, Nostoc and LPP group representatives were also found, as well as Fischerella. On the aerial roots of P. amabilis and D. phalaenopsis grown in a greenhouse simulating the climate of moist tropical forest, cyanobacteria were represented by Nostoc, LPP group, and Scytonema in D. phalaenopsis and by Nostoc, Scytonema, Calothrix, Spirulina, Oscillatoria, and the LPP group in P. amabilis. For D. moschatum, the spectra of cyanobacteria populating the substrate root rhizoplane and the substrate (pine bark) were compared. In the parenchyma of the aerial roots of P. amabilis, fungal hyphae and/or their half-degraded remains were detected, which testifies to the presence of mycorrhizal fungi in this plant. This phenomenon is attributed to the presence of a sheath formed by cyanobacteria and serving as a substrate for fungi. |
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Keywords: | epiphytic orchids sheath associative cyanobacteria |
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