Alternative pathway activities in aged potato tuber slices measured by three methods |
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| Authors: | Z Hou G Zhou Y Kong W Liang L Zhang |
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| Institution: | (1) State Key Laboratory of Arid Agroecology, School of Life Sciences, Lanzhou University, 730000 Lanzhou, China;(2) Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Academy of Sciences of China, 730000, China;(3) Institute of Genetics and Developmental Biology, Academy of Sciences of China, 100080 Beijing, China |
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| Abstract: | To find a simple and reliable oxygen electrode-based method to estimate the values of alternative pathway activity ( V
alt) and its contribution to total respiration V
alt/V
t) in aged potato (Solanum tuberosum L.) tuber slices, we compared conventional hydroxamate-inhibiting method, improved hydroxamate-inhibiting method with 2,6-dichlorophenol indophenol (DCPIP), and the oxygen isotope discrimination (OID) method. The values of V
alt and V
alt/V
t obtained with an improved hydroxamate-inhibiting method with DCPIP in 12-h- and 24-h-aged slices were about twice higher than those with the conventional hydroxamate-inhibiting method. Only a relatively small difference in the values of V
alt and V
alt/V
t obtained by the OID method and the improved hydroxamate-inhibiting method with DCPIP in 12-h and 24-h-aged slices was observed. These results indicated that the improved hydroxamate-inhibiting method with DCPIP could be considered as a new, simple, and reliable technique for the noninvasive assay of the AP activity.From Fiziologiya Rastenii, Vol. 52, No. 2, 2005, pp. 311–315.Original English Text Copyright © 2005 by Hou, Zhou, Kong, Liang, Zhang.This article was submitted by the authors in English.This revised version was published online in April 2005 with a corrected cover date. |
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| Keywords: | Solanum tuberosum alternative pathway cytochrome pathway oxygen isotope discrimination potato tuber slices |
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