肝硬化门脉高压症大鼠模型制作方法的探讨

目的：建立稳定可靠的肝硬化门脉高压大鼠动物模型。材料与方法：50只雄性sD大鼠，随机分为3组。正常对照组10只，行假手术，给予正常饮食。肝硬化A组20只，先行左肾上腺静脉结扎，然后给予初始浓度为0．03％的硫代乙酰胺（thioacetamide，TAA）溶液作为饮用水并根据大鼠体重变化调节给药浓度。肝硬化B组20只，行假手术，给予固定浓度为0．03％TAA溶液。给药时间为14周。结果：肝硬化A组大鼠死亡率为0，肝硬化形成率达到100％。肝硬化B组大鼠死亡率15％，肝硬化形成率75％。肝硬化A组大鼠的门静脉压力明显高于肝硬化B组和对照组。结论：采用左肾上腺静脉结扎并根据体重变化调节TAA给药浓度可建立稳定可靠的肝硬化门脉高压大鼠动物模型。

Investigation of modeling portal hypertension rats with cirrhosis

Objective:To establish a reliable portal hypertension rat model with cirrhosis.Materials and methods:Fifty male SDrats were randomly divided into three groups:group A,B and control group.The left adrenal vein of group A(n=20) was ligated bofore receiving continuous administration of thioacetamide(TAA) in the drinking water.And the concentration d TAA was adjusted according to weekly weight changes of the rats,with the initial concentration 0.03%.Group B(n=20) was given sham operation with the fixed concentration of TAA 0. 03%.The control group (n=10) was accepted sham operation and was supplied with normal water.Results:The mortality in group B was 15% and the rate of cirrhosis formed was only 75%.In contrast,there was no death and cirrhosis formed was 100% of the rats in group A.The portal venous pressure of the rats in group A was higher than that of group B and the control group.Conclusions:The method of combining ligation of the left adrenal vein with adjustment of concentration of TAA in the drinking water according to weight changes of the rats can establish a reliable rat model of portal hypertension with cirrhosis.