An improved enrichment broth for isolation of Escherichia coli O157, with specific reference to starved cells,from radish sprouts |
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Authors: | Sata Shin Fujisawa Tomohiko Osawa Ro Iguchi Atsushi Yamai Shiro Shimada Toshio |
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Institution: | Kanagawa Prefectural Public Health Laboratory, Asahi-ku, Yokohama 241-0815, Japan. |
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Abstract: | An enrichment broth was developed for the efficient isolation of Escherichia coli O157 from radish sprouts. The broth was buffered peptone water containing 0.5% sodium thioglycolate (STG-BPW), which was designed to allow growth of E. coli O157 in starved and unstarved states. However, this medium suppressed the growth of non-carbohydrate-fermenting obligate aerobes whose colonial appearance on sorbitol MacConkey agar containing cefixime and tellurite (CT-SMAC) resembled that of E. coli O157. Both starved and unstarved cells of E. coli O157 experimentally inoculated into radish sprouts were successfully recovered with STG-BPW enrichment in all cases, most of which showed marked disappearance of E. coli O157-like colonies on CT-SMAC. |
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