Presence of glucagon-(1-21)-Like immunoreactive substance in the dog small intestinal mucosa |
| |
Authors: | H Itoh M Namba N Watanabe T Ohara T Matsuyama R Komatsu Y Shinomura N Kono S Tarui |
| |
Institution: | Second Department of Internal Medicine, Osaka University Medical School, Japan. |
| |
Abstract: | By using an antiserum (K291) specifically directed to the C-terminal of glucagon-(1-21)-peptide, we demonstrated the presence of glucagon-(1-21)-like immunoreactivity (G21-IR) in the dog intestine. G21-IR was found to be widely distributed throughout the small intestine and colon in parallel with the distribution of glucagon-like immunoreactivity (GLI), measured by N-terminal glucagon antiserum (OAL196). The subsequent analyses by gel filtration and three HPLC columns (reverse phase, ion exchange and further reverse phase columns) showed that G21-IR consisted of three main peaks, and the smallest molecular form of G21-IR is identical to glucagon-(1-21)-peptide. |
| |
Keywords: | |
|
|