WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells |
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Authors: | Kho Yoonjung Kim Sungchan Yoon Byung Sun Moon Jai-Hee Kwak Sungwook Park Gyuman Woo Junghee Oh Sejong Hong Kichang Kim Saehun Kim Hyunggee You Seungkwon Choi Yunjaie |
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Institution: | School of Agricultural and Biotechnology, College of Agriculture and Life Sciences Seoul National University, Seoul, South Korea. |
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Abstract: | In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNFalpha treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNFalpha induced WDNM1 expression showed the NFkappaB-dependent mechanism since it's expression was abrogated in IkappaBalphaM (super-repressor of NFkappaB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNFalpha-NFkappaB signal pathway, is associated with HC11 cell apoptosis. |
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