Characterization ofClostridium bifermentans and its biotransformation of 2,4,6-trinitrotoluene (TNT) and 1,3,5-triaza-1,3,5-trinitrocyclohexane (RDX) |
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Authors: | K. M. Regan R. L. Crawford |
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Affiliation: | (1) Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, 83844-1052 Moscow, Idaho;(2) Center for Hazardous Waste Remediation Research, University of Idaho, 83844-1052 Moscow, Idaho |
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Abstract: | Summary We have determined that an organism able to degrade both RDX and TNT in a pure culture is a strain ofClostridium bifermentans. The consortium from which this organism is derived also degrades these compounds, and we suspect thatC. bifermentans is also the responsible organism within that consortium. The bioconversion of RDX and TNT occurs under anaerobic conditions both in the consortium and in pure culture without the need of an added reductant. The presence of co-metabolites speeded these biotransformations. |
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