Mechanism of nitrite inhibition of cellular respiration inPseudomonas aeruginosa |
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Authors: | Dr. Tsanyen Yang |
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Affiliation: | (1) Department of Biological Sciences, Bowling Green State University, 43403 Bowling Green, Ohio, USA |
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Abstract: | One of the principal mechanisms of nitrite inhibition of cellular respiration has been considered to be the interference with the action of iron-containing enzymes. In procaryotic systems, the effect of nitrite on cellular metabolism remains unclear. This study provides evidence which shows a direct inhibition by a low concentration of nitrite on a highly purified oxidase inPseudomonas aeruginosa. The inhibition pattern was observed and was consistent at cellular, electron-transport membranous, and enzymic (oxidase) levels. This implies that the mechanism of nitrite inhibition on bacterial respiration is due to a direct inhibition at the terminal site of oxygen reduction. The uncompetitive inhibition pattern shown by nitrite strongly suggested a mechanism quite different from those of classic cytochrome oxidase inhibitors such as cyanide, azide, and carbon monoxide. |
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