New RAPD markers of tomato spotted wilt virus (TSWV) resistance in Lycopersicon esculentum Mill |
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Authors: | Mieczysław Śmiech Zbigniew Rusinowski Stefan Malepszy Katarzyna Niemirowicz-Szczytt |
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Affiliation: | (1) Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, Nowoursynowska Str.166, 02-787 Warsaw, Poland |
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Abstract: | The selection of TSWV resistant individuals can be facilitated by molecular markers. RAPD analysis was carried out on three forms (Stevens × Rodade — resistant; Rey de los Tempranos — moderately tolerant; Potentat — susceptible) with the use of 271 primers. Out of 271 primers 28 generated stable polymorphism and so they were tested for linkage to resistance gene. Bulk segregant analysis (BSA) was applied to F2 segregating progeny developed from resistant × susceptible parents. As a result, 5 primers enabling us to distinguish between resistant and susceptible forms were detected. Only one of them had previously been reported by Chague et al. (1996). The analysis should be repeated on a larger population to confirm the results obtained. |
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Keywords: | RAPD Sw-5 tomato TSWV |
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