Characterization and fine mapping of <Emphasis Type="Italic">RppQ</Emphasis>, a resistance gene to southern corn rust in maize |
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Authors: | Chunjiang Zhou Cuixia Chen Pengxiu Cao Suowei Wu Jianwei Sun Demin Jin Bin Wang |
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Institution: | (1) College of Life Science, Hebei Normal University, Shijiazhuang, Hebei, 050016, China;(2) State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China;(3) College of Agronomy, Shandong Agricultural University, Taian, Shandong, 271018, China |
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Abstract: | Southern corn rust (SCR) is a fungal disease caused by Puccinia polysora Underw, which can infect maize and may result in substantial yield losses in maize production. The maize inbred line Qi319
carries the SCR resistance gene RppQ. In order to identify molecular markers linked to the RppQ gene, several techniques were utilized including random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and
amplified fragment length polymorphism (AFLP). In addition, sequence characterized amplified region (SCAR) techniques combined
with bulked segregant analysis (BSA) were used. Seven RAPD markers, eight SSR markers, and sixty-three AFLP primer combinations
amplified polymorphisms between two parents and two bulk populations. A large F2 population was used for genetic analysis and for fine mapping of the RppQ gene region. One AFLP polymorphic band, M-CAA/E-AGC324, was converted to a SCAR marker, MA7, which was mapped to a position 0.46 cM from RppQ. Finally, the RppQ gene was mapped between the SCAR marker MA7 and the AFLP marker M-CCG/E-AGA157 with distances of 0.46 and 1.71 cM, respectively. |
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Keywords: | Maize (Zea mays L ) Southern corn rust Molecular marker Fine mapping |
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