Enhanced multiplication and progesterone production by porcine granulosa cells cultured in a serum-free medium |
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| Authors: | S Stok?osowa E Gregoraszczuk E Hole?ková J Michl |
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| Institution: | 1. Laboratory of Animal Endocrinology and Tissue Culture, Department of Animal Physiology, Institute of Zoology, Jagellonian University, 30-060 Krakow, Poland;2. Institute of Physiology, Czechoslovak Academy of Sciences, 14220 Prague, Czechoslovakia |
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| Abstract: | Granulosa cells harvested from pro-estrous follicles of porcine ovary were grown in medium 199 supplemented with 0.4, 1 and 10% of growth-promoting calf serum proteins (GPP), and their multiplication and hormonal activity were compared with those of sister cultures carried in medium 199 supplemented with 10% calf serum. The medium containing the growth-promoting proteins was always superior to the whole-serum medium with regard to cell multiplication, activity of Δ5,3 hydroxysteroid dehydrogenase detected histochemically in the cells, and production of progesterone estimated by radioimmunoassay in the medium. It was inferior when it came to estrogen secretion in the beginning of the cultivation when calculated on a per cell basis. |
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