Production of pectolytic enzymes fromErwinia grown on different carbon sources |
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| Authors: | V. E. Shevchik A. N. Evtushenkov H. V. Babitskaya Y. K. Fomichev |
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| Affiliation: | (1) Department of Microbiology, Byelorussian State University, Leninsky pr. 4, 220080 Minsk, Byelorussia |
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| Abstract: | A quantitative analysis of pectolytic enzymes (polygalacturonase (PG), pectin methyl esterase (PME) and six isoenzymes of pectate lyase (PL)) produced byErwinia bacteria in the presence of diverse carbon sources was made by preparative electrophoresis. Synthesis of each of these enzymes was regulated independently; different induction and repression ratios (about 10- to 1000-fold) were observed for diverse PL isoenzymes, PG and PME. The possibility of using specially constructed media for the production of pectinase complexes with a specific spectra of pectolytic enzymes has been demonstrated. |
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| Keywords: | Erwinia pectate lyase pectin polygalacturonase methyl esterase |
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