Hypobaric hypoxia affects endogenous levels of alpha-keto acids in murine heart ventricles |
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Authors: | Mühling Jörg Paddenberg Renate Hempelmann Gunter Kummer Wolfgang |
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Institution: | Clinic of Anaesthesiology, Intensive Care Medicine, Pain Therapy and Palliative Medicine, Justus-Liebig-University, Giessen, Germany. |
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Abstract: | Alpha-keto acids have recently been identified as potent regulators of cellular adaptations to hypoxia. Their actual intracellular concentrations under such conditions are unknown. Here, we determined concentrations of alpha-ketobutyrate, alpha-ketoglutarate, alpha-ketoisocaproate, alpha-ketoisovalerate, alpha-keto-beta-methylvalerate, phenylpyruvate, and pyruvate by a recently developed ultra-sensitive fluorescence HPLC method in ventricular myocardium of mice exposed to hypobaric hypoxia for up to 3 weeks. We observed characteristic alterations of cardiac alpha-keto acid concentrations that are specific for individual alpha-keto acids, show significant side differences (right versus left ventricles), and are suited to trigger some of the cardiac metabolic and structural adaptations to chronic hypoxia. |
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Keywords: | α-Keto acids α-Ketoglutarate Pyruvate Hypoxia Heart |
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