Spectrophotometric measurement of carotenes, xanthophylls, and chlorophylls in extracts from plant seeds |
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| Authors: | O. V. Bulda V. V. Rassadina H. N. Alekseichuk N. A. Laman |
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| Affiliation: | (1) Kuprevich Institute of Experimental Botany, National Academy of Sciences of Belarus, Akademicheskaya ul. 27, Minsk, 220072, Belarus;(2) Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, Minsk, Belarus |
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| Abstract: | The method of spectrophotometric measurement of carotenoid and chlorophyll content in extracts from plant seeds was modified. The pigments were extracted with a mixture of petroleum ether (PE) and tetrahydrofuran (THF) (PE to THF ratio 4: 1). Equations for calculations of β-carotene, lutein, chlorophyll a and b content in PE: THF mixture were obtained using specific absorption coefficients of solutions of particular pigments. It was shown that subtraction of chlorophyll contribution from the absorption spectrum of seed extracts yields spectra, which could be used in some cases for calculation of carotene and xanthophyll contents. |
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| Keywords: | plants seeds carotene xanthophylls chlorophylls a and b spectrophotometry equations for calculation of pigment content |
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