| 激光辐照葡萄愈伤组织的过氧化物酶同工酶分析 |
| |
| 引用本文： | 张克忠,刘恩泉.激光辐照葡萄愈伤组织的过氧化物酶同工酶分析[J].激光生物学报,1994,3(2):453-456. |
| |
| 作者姓名： | 张克忠 刘恩泉 |
| |
| 作者单位： | 山东大学生物系，山东大学光学系 |
| |
| 摘 要： | 利用法国产ＤＡＴＡＣＨＲＯＭ—５０００型染料脉冲激光器处理葡萄“胜利”品种的愈伤组织，用聚丙烯酰胺凝胶电泳法（ＰＡＧＥ）分离过氧化物酶同工酶，研究了激光辐照对葡萄生长发育和过氧化物酶同工酶表达的影响，发现：适当剂量的激光辐照能使葡萄愈伤组织明显提前分化；激光能够影响葡萄过氧化物酶同工酶基因的调控，适当剂量的激光能够促进该基因的表达；激光处理的愈伤组织在不同的生长发育时期与对照相比，过氧化物酶同工酶在数目和活性上有明显增加。
|
| 关 键 词： | 激光 葡萄 愈伤组织 过氧化物酶 |
ANALYSIS OF PEROXIDASE ISOENZYMES OF GRAPEVINE CALLUS IRRADIATED BY LASER |
| |
| Zhang Kezhong, Bao Xuezhen.ANALYSIS OF PEROXIDASE ISOENZYMES OF GRAPEVINE CALLUS IRRADIATED BY LASER[J].ACTA Laser Biology Sinica,1994,3(2):453-456. |
| |
| Authors: | Zhang Kezhong Bao Xuezhen |
| |
| Abstract: | Grapevine callus of Vitis Vinifera L. CV. Triumph were irradiated by using DATACHROM 5000 type laser machine made in France. Peroxidase isoenzymes were separated by polyacrylamide gel elec-trophorosis. We found that:callus irradiated with appropriate dose of laser could differentiate ahead of timeobviously;laser irradiation could affect the regulation of peroxidase gene and appropriate dose of laser couldpromote the expression of peroxidase gene;compared with the control group,the kinds and activity of perox-idase isoenzymes of grapevine callus treated with laser were taised Obviously. |
| |
| Keywords: | Laser Grapevine callus Peroxidase isoenzyme Polyacrylamide gel electrophorosis(PAGE) |
| 本文献已被 CNKI 维普 等数据库收录！ |
