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NaCl胁迫对大麦细胞分裂及染色体行为的影响
引用本文:仪慧兰,张自立.NaCl胁迫对大麦细胞分裂及染色体行为的影响[J].遗传,2001,23(1):29-32.
作者姓名:仪慧兰  张自立
作者单位:1.山西大学生命科学系,太原 030006; 2.南开大学生命科学院,天津 300071 1.Department of Life Science,Shanxi University,Taiyuan 030006,China; 2.Institute of Life Science,Nankai University,Tianjin 300071,Chnia
基金项目:国家自然科学基金(39070513)和山西省青年科学基金(981038)资助
摘    要:NaCl溶液培养导致大麦幼苗根尖细胞有丝分裂指数下降,细胞姐妹染色单体交换(SCE)频率增高,且诱发包括染色体断片、后期染色体桥、不均等分裂及间期细胞微核等的染色体行为异常。细胞平均SCE频率及异常分裂细胞的比率与NaCl浓度和作用时间呈正相关。结果提示:NaCl浓度高或作用时间较长时对大麦细胞具有遗传学毒性。 Abstract:The effects of NaCl solution on chromosome behavior and sister chromatid exchanges(SCE)of barley were studied.Abnormal chromosome behavior including chromosome fragmentation,micronuclei,anaphase bridges and unequal split was found in root tip cells of Hordeum vulgare seedlings.Mitotic index decreased but SCE frequency increased significantly when barley incubated with NaCl solution.The effects of NaCl solution depended on its concentration and treatment duration.The higher the treated concentration was,the higher the ratio of chromosomal aberration was.The longer the treatment duration was,the higher the degree of the effects was.The results showed that NaCl solution was genotoxic when the concentration was higher and the treated time was longer.

关 键 词:Hordeum  vulgare  染色体行为异常  姐妹染色单体交换  Keywords  NaCl  大麦  
文章编号:0253-9772(2001)01-0029-04
修稿时间:2000年1月31日

Cell Division and Chromosome Behavior of Hordeum vulgare Seedlings under Salt Stress
YI Hui-lan,ZHANG Zi-Li.Cell Division and Chromosome Behavior of Hordeum vulgare Seedlings under Salt Stress[J].Hereditas,2001,23(1):29-32.
Authors:YI Hui-lan  ZHANG Zi-Li
Abstract:The effects of NaCl solution on chromosome behavior and sister chromatid exchanges(SCE)of barley were studied.Abnormal chromosome behavior including chromosome fragmentation,micronuclei,anaphase bridges and unequal split was found in root tip cells of Hordeum vulgare seedlings.Mitotic index decreased but SCE frequency increased significantly when barley incubated with NaCl solution.The effects of NaCl solution depended on its concentration and treatment duration.The higher the treated concentration was,the higher the ratio of chromosomal aberration was.The longer the treatment duration was,the higher the degree of the effects was.The results showed that NaCl solution was genotoxic when the concentration was higher and the treated time was longer.
Keywords:Hordeum vulgare  NaCl  abnormal chromosome behavior  sister chromatid exchange
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