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Synthesis and matrix assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry study of phosphopeptide |
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| Authors: | Shi-Zhong Luo Yan-Mei Li Zhong-Zhou Chen Hiroshi Abe Li-Ping Cui Hiroshi Nakanishi Xu-Rong Qin and Yu-Fen Zhao |
| Institution: | (1) Bioorganic Phosphorus Chemistry Laboratory, Department of Chemistry, School of Life Science and Engineering, Tsinghua University, Beijing, P.R. China;(2) Biological Information Research Center, National Institute Advanced Industry Science and Technology AIST, Central 6, 1-1, Higashi, Tsukuba, Japan |
| Abstract: | The symmetry phosphoramidite reagents were synthesized via atwo-step route and used for the `one pot' synthesis of thephosphoserine building block Fmoc-Ser(PO(OBzl)OH)-OH. Theprocedure is simple and rapid, and product is obtained in highyield. When using this building block, the phosphopeptide(RKGS(PO3H2)SSNEPSSDSLSSPTLLAL) related to theC-terminal of c-Fos protein was synthesized manually byFmoc/t-Bu procedure and characterized by matrix assisted laserdesorption/ionization time of flight (MALDI-TOF) massspectrometry. In the fragment ion of phosphopeptide in theMALDI-TOF mass spectrometry with a curved field reflection, theacquired information can be used to identify the sequences andthe phosphorylation sites of the peptide. |
| Keywords: | building block curved field reflection matrix assisted laser desorption/ionization time of flight one pot phosphopeptide solid-phase peptide synthesis |
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