糜酶转基因小鼠心脏组织中基质金属蛋白酶9的活化

为研究糜酶在心脏中的功能 ,用明胶酶谱法和放免法检测了糜酶转基因小鼠心脏组织中基质金属蛋白酶及糜酶的活力 .糜酶转基因小鼠心脏组织中糜酶样活力较转基因阴性小鼠升高了约80 % ;而其心脏匀浆液凝胶酶谱分析结果显示在 92 k D处明胶酶活力也升高约 30 % ;经 Western印迹鉴定为基质金属蛋白酶 9,而在蛋白水平上与转基因阴性小鼠无显著差异 .结果提示 ,糜酶转基因小鼠心脏组织中糜酶活力的升高可活化基质金属蛋白酶 9,从而影响心脏胶原代谢 .

Activation of Matrix Metalloprotease 9 in the Heart Tissue of Chymase Transgenic Mice

To study the function of chymase in the heart,the enzymograghy and radioimmu noassay were used to measure the activities of matrix metalloproteases(MMPs) and chymase.Compared with the negative transgenic mice,the activity of chymase in the heart tissue of chymase transgenic mice increased about 80% and the activity of a 92 kD MMP increased about 30%.By Western blot,the 92 kD MMP was identified as MMP 9 but there was no marked difference at protein level between negative and positive transgenic mice.The results suggested that the elevation in chymase activity could activate MMP 9 and affect the collagen metabolism in the hearts of transgenic mice.