The phosphoenolpyruvate: methyl-α-D-glucoside phosphotransferase system in Bacillus subtilis Marburg: kinetic studies of enzyme II and evidence for a phosphoryl enzyme II intermediate

The Enzyme II complex catalyzing the phosphoryl transfer from P-HPr to sugar in the inducible methyl-α-D-glucoside: phosphotransferase system in Bacillus subtilis acts according to a ping-pong mechanism, implying a phosphorylated Enzyme II intermediate. This result is supported by the demonstration of a specific transphosphorylation between [¹⁴C] αMG and glucose-6-phosphate in the presence of an induced Enzyme II preparation.