Effect of lactic acid on growth and butanediol production byKlebsiella oxytoca |
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Authors: | N Qureshi M Cheryan |
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Institution: | (1) Biochemical Engineering Laboratory, University of Illinois, 382 A.E.S. Building, 1304 W. Pennsylvania Avenue, 61801 Urbana, IL |
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Abstract: | Summary Production of 2,3-butanediol byKlebsiella oxytoca was enhanced in the presence of low levels (<8 g/l) of added sodium lactate. Cell growth was inhibited, however, and essentially stopped above 15 g/l added lactate. Levels of by-products (acetic acid and ethanol) were also higher. With 3 g/l lactate and an initial glucose level of 98 g/l, butanediol concentration and productivity increased 164% with 98% utilization of glucose. With high glucose concentration (219 g/l), addition of 2.64 g/l lactate after the growth phase resulted in 81 g/l butanediol, with a productivity of 0.65 g/l/h and 71% glucose utilization. |
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Keywords: | Klebsiella oxytoca 2 3-Butanediol Lactic acid Ethanol Glucose |
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