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A practical synthesis of capped 4-methylumbelliferyl hyaluronan disaccharides and tetrasaccharides as potential hyaluronidase substrates |
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| Authors: | Gold Henrik Munneke Stefan Dinkelaar Jasper Overkleeft Herman S Aerts Johannes M F G Codée Jeroen D C van der Marel Gijs A |
| Affiliation: | aLeiden Institute of Chemistry, Leiden University, Gorlaeus Laboratories, Einsteinweg 55, 2300 RA Leiden, The Netherlands;bDepartment of Medical Biochemistry, Academic Medical Center, University of Amsterdam, Germany |
| Abstract: | The synthesis of hyaluronan dimers and tetramers equipped with a 4-methylumbelliferyl group at the reducing end to potentially allow monitoring of hyaluronidase activities is described. The 4-OH at the non-reducing glucuronate in the presented series is either removed or methylated to prohibit transglycosylase reactions, leading to a total of four probes. |
| Keywords: | Hyaluronan Hyaluronidase Fluorogenic substrate Glycosylation Barton deoxygenation |
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