Elucidation of the structure and characterization of the gene cluster of the O-antigen of Cronobacter sakazakii G2592, the reference strain of C. sakazakii O7 serotype |
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| Authors: | Arbatsky Nikolay P Wang Min Daeva Elena D Shashkov Alexander S Feng Lu Knirel Yuriy A Wang Lei |
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| Institution: | aZelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation;bTEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, PR China;cTianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, TEDA, Tianjin 300457, PR China |
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| Abstract: | The O-specific polysaccharide from the lipopolysaccharide of Cronobacter sakazakii G2592 was studied by sugar analysis along with 1D and 2D 1H and 13C NMR spectroscopy, and the following structure of the pentasaccharide repeating unit was established:This structure is unique among the known bacterial polysaccharide structures, which is in accord with classification of strain G2592 into a new C. sakazakii serotype, O7. It is in agreement with the O-antigen gene cluster of this strain, which was found between the housekeeping genes JUMPStart and gnd and characterized by sequencing and tentative assignment of the gene functions. |
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| Keywords: | Cronobacter sakazakii O-Specific polysaccharide Lipopolysaccharide Bacterial polysaccharide structure O-Antigen gene cluster |
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