New method for peptide desorption from abundant blood proteins for plasma/serum peptidome analyses by mass spectrometry |
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Authors: | Ziganshin Rustam Arapidi Georgii Azarkin Igor Zaryadieva Elena Alexeev Dmitry Govorun Vadim Ivanov Vadim |
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Institution: | M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation. rustam.ziganshin@gmail.com |
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Abstract: | This report describes a new method for desorption of low-molecular weight (LMW) peptides from abundant blood proteins for use in subsequent mass spectrometry analyses. Heating of diluted blood serum to 98°C for 15min resulted in dissociation of LMW peptides from the most abundant blood proteins. Application of blood plasma/serum fractionation using magnetic beads with a functionalized surface followed by heating of the resultant fractions significantly increases the number of LMW peptides detected by MALDI-TOF MS, enhances the general reproducibility of mass spectrometry profiles and considerably increases the number of identified blood serum peptides by LC-MS/MS using an Agilent 6520 Accurate-Mass Q-TOF. |
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