Abstract: | In the presence of Mg2+ the ecto-(nucleoside diphosphatase) on intact vascular endothelial or smooth muscle cells in culture selectively catabolizes the PS diastereoisomer of adenosine 5'-alpha-thio]diphosphate, (PS)-ADP alpha S], and the ecto-(nucleoside triphosphatase) selectively catabolizes the PS isomer of adenosine 5'-beta-thio]triphosphate, (PR)-ATPbeta S], but exhibits no selectivity towards ATPalpha S] isomers. In the presence of Cd2+ selectivity to ADPalpha S] and to ATPbeta S] isomers is reversed; in the presence of Co2+, selectivity is lost. We conclude that each enzyme preferentially recognises the lambda (screw-sense) bidentate Mg(II)-nucleotide complex at its active site. |