Characterization of the relA gene of Porphyromonas gingivalis |
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Authors: | Sen K Hayashi J Kuramitsu H K |
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Affiliation: | Departments of Oral Biology, State University of New York, Buffalo, New York 14214, USA. |
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Abstract: | Based upon the nucleotide sequence of the relA gene from Escherichia coli, a gene fragment corresponding to the homologous gene from the pathogenic oral bacterium Porphyromonas gingivalis 381 was isolated by PCR and utilized to construct a relA mutant. The mutant, KS7, was defective in ribosome-mediated ppGpp formation and also in the stringent response. |
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