Laccase from Melanocarpus albomyces binds effectively to cellulose |
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Authors: | Kiiskinen Laura-Leena Palonen Hetti Linder Markus Viikari Liisa Kruus Kristiina |
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Institution: | VTT Biotechnology, P.O. Box 1500, Fin-02044 VTT, Finland. |
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Abstract: | A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one-site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non-specific protein or 0.1-0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases. |
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