Genetic Mapping of a Defective Bacteriophage on the Chromosome of Bacillus subtilis 168 |
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| Authors: | Anthony J Garro Hyam Leffert and Julius Marmur |
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| Institution: | Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461 |
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| Abstract: | A genetic marker responsible for the killing activity of PBSX, a defective phage carried by Bacillus subtilis 168, has been located on the bacterial chromosome. Two mutant strains of B. subtilis 168, which produced tailless phage particles upon mitomycin C induction, were shown to carry lesions, designated xtl-1 and xtl-2, which were linked by transformation and PBS1-mediated transduction to metC. The link-age relationship between xtl and adjacent auxotrophic markers was determined by three-factor PBS1 transduction, the suggested order of markers being argO 1 metA metC xtl. |
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