Identification of the NH2-terminal blocking group of calcineurin B as myristic acid |
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| Authors: | Alastair Aitken Philip Cohen Sitthivet Santikarn Dudley H. Williams A.Graham Calder Alistair Smith Claude B. Klee |
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| Affiliation: | Department of Biochemistry, Mount Sinai School of Medicine of the City University of New York, One Gustave L. Levy Place, New York, NY 10029, USA |
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| Abstract: | The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-Gly---Asn---Glu---Ala-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group. |
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| Keywords: | Protein phosphatase Ca2+ Calmodulin Mass spectrometry Fatty acids High-performance liquid chromatography |
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