Development of an optical sensor for chlortetracycline detection based on the fluorescence quenching of l‐tryptophan |
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Authors: | Hui Zhang Hongyun Chen Shuang Pan Huan Yang Jingjing Yan Xiaoli Hu |
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Affiliation: | Key Laboratory of Luminescent and Real‐Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China |
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Abstract: | A simple and selective spectrofluorimetric method for the detection of chlortetracycline (CTC) was studied. In pH 7.4 buffer medium l ‐tryptophan (l ‐Trp), applied as the fluorescence probe, interacted with CTC resulting in fluorescence quenching of the probe. CTC was detected with maximum excitation and emission wavelengths at λex/λem = 275/350 nm. Notably, quenching of fluorescence intensities was positively proportional to the CTC concentration over the range of 0.65–30 μmol L?1 and the limit of detection was 0.2 μmol L?1. Effect of temperature shown in Stern?Volmer plots, absorption spectra and fluorescence lifetime determination, indicated that fluorescence quenching of l ‐Trp by CTC was mainly by static quenching. The proposed study used practical samples analysis satisfactorily. |
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Keywords: | chlortetracycline detection fluorescence quenching l‐tryptophan |
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