A rhodamine‐triazole fluorescent chemodosimeter for Cu2+ detection and its application in bioimaging |
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| Authors: | Kanokorn Wechakorn Samran Prabpai Kanoknetr Suksen Phongthon Kanjanasirirat Yongyut Pewkliang Suparerk Borwornpinyo Palangpon Kongsaeree |
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| Affiliation: | 1. Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand;2. Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok, Thailand;3. Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand;4. Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, Bangkok, Thailand;5. Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand |
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| Abstract: | A rhodamine‐based fluorescent chemodosimeter rhodamine hydrazide‐triazole (RHT) tethered with a triazole moiety was developed for Cu2+ detection. In aqueous medium, the RHT probe exhibited high selectivity and sensitivity toward Cu2+ among other metal ions. The addition of Cu2+ triggered a fluorescence emission of RHT by 384‐fold (Φ = 0.33) based on a ring‐opening process and a subsequent hydrolysis reaction. Moreover, RHT also showed a selective colorimetric response toward Cu2+ from colorless solution to pink, readily observed with the naked eye. The limit of detection of RHT for Cu2+ was calculated to be 1 nM (0.06 ppb). RHT was successfully demonstrated to detect Cu2+ in Chang liver cells by confocal fluorescence microscopy. |
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| Keywords: | 1,2,3‐triazole bioimaging Cu2+‐selective chemodosimeter fluorescence detection rhodamine |
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