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Probabilistic base calling of Solexa sequencing data |
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| Authors: | Jacques Rougemont Arnaud Amzallag Christian Iseli Laurent Farinelli Ioannis Xenarios Felix Naef |
| Institution: | 1.School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL),Lausanne,Switzerland;2.Ludwig Institute for Cancer Research (LICR), Batiment Génopode,Université de Lausanne,Lausanne,Switzerland;3.Swiss Institute of Bioinformatics (SIB), Batiment Génopode,Université de Lausanne,Lausanne,Switzerland;4.Vital-IT, Batiment Génopode,Université de Lausanne,Lausanne,Switzerland;5.Fasteris SA,Plan-les-Ouates,Switzerland |
| Abstract: | BackgroundSolexa/Illumina short-read ultra-high throughput DNA sequencing technology produces millions of short tags (up to 36 bases) by parallel sequencing-by-synthesis of DNA colonies. The processing and statistical analysis of such high-throughput data poses new challenges; currently a fair proportion of the tags are routinely discarded due to an inability to match them to a reference sequence, thereby reducing the effective throughput of the technology. |
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