Irisin ameliorates angiotensin II-induced cardiomyocyte apoptosis through autophagy |
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Authors: | Ruli Li Xiaoxiao Wang Sisi Wu Yao Wu Hongying Chen Juanjuan Xin He Li Jie Lan Kunyue Xue Xue Li Caili Zhuo Jinhan He Chao-Shu Tang Wei Jiang |
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Affiliation: | 1. Department of Pharmacy, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China;2. Department of Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing Cancer Institute, Chongqing Cancer Hospital, Chongqing, China Li and Wang 3. are co-first authors and contributed equally to this work.;4. Deparment of Molecular Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China;5. Department of Pharmacy, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China;6. Department of Pathology and Physiology, Peking University Health Science Center, Beijing, People's Republic of China |
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Abstract: | Cardiac hypertrophy is the main cause of heart failure and sudden death in patients. But the pathogenesis is unclear. Angiotensin II may contribute to cardiac hypertrophy in response to pressure overload. In angiotensin II-treated cardiomyocytes, there is a larger cross-sectional area, more apoptosis cells, and a reduction of irisin expression. An increase in P62, an autophagy flux index, as well as LC3II, were observed in cardiomyocytes after angiotensin II-induced injury. Surprisely, irisin supplementation increased LC3II expression and decreased P62 expression, consisted of results of RFP-GFP-LC3B adenovirus transfection, and reduced cardiomyocyte apoptosis, meanwhile, the protection of irisin was reversed by the autophagy inhibitor 3-methyladenine. In animal experiments, overexpression of irisin reduced cardiomyocyte apoptosis and alleviated myocardial hypertrophy caused by pressure overload. The above results indicate that irisin-induced protective autophagy and alleviated the apoptosis signaling pathway in cardiomyocytes, consequently reducing cardiomyocyte apoptosis after angiotensin II-induced injury. Hence, increasing irisin expression may be a new way to improve cardiac function and quality of life in patients with cardiac hypertrophy. |
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Keywords: | angiotensin II apoptosis autophagy cardiac hypertrophy irisin |
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