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miR-548c-5p inhibits colorectal cancer cell proliferation by targeting PGK1 |
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| Authors: | Jianxin Ge Jun Li Su Na Pingping Wang Guifeng Zhao Xiaoyan Zhang |
| Institution: | 1. Department of Gastroenterology, People's hospital of Jiangbei, Nanjing, China
These authors have contributed equally to this study.;2. Department of General Surgery, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, China These authors have contributed equally to this study.;3. Department of Oncology, People's Hospital of Rizhao, Rizhao, China These authors have contributed equally to this study.;4. Department of Gynecology and Obstetrics, Weifang Hospital of Maternal and Child Health, Weifang, China;5. Department of Antenatal Diagnosis, People's Hospital of Weifang, Weifang, China;6. Operating Room, People's Hospital of Zhucheng, Zhucheng, China |
| Abstract: | Accumulating studies have implicated that microRNAs (miRNAs) are involved in the pathogenesis of colorectal cancer (CRC). However, the role of miR-548c-5p, a novel identified miRNA in malignancies, in colorectal carcinogenesis remains largely unknown. The present study is aimed to investigate the effect and molecular mechanism of miR-548c-5p in CRC by a sequence of cellular experiments. miR-548c-5p was significantly downregulated, whereas phosphoglycerate kinase 1 (PGK1), a key enzyme for glycolysis, was obviously upregulated in peripheral blood mononuclear cells and cancer tissues from patients with CRC. Besides, miR-548c-5p and PGK1 were negatively associated with each other. The luciferase reporter assay revealed that PGK1 was a targeted gene of miR-548c-5p. Moreover, the proliferation and generation of inflammatory cytokines (TNF-α and IL-6) were significantly inhibited in miR-548c-5p-overexpressed SW480 CRC cells stimulated by lipopolysaccharide (LPS). Accordingly, miR-548c-5p may serve as a cancer suppressor in CRC by targeting PGK1. |
| Keywords: | colorectal cancer inflammation miR-548c-5p phosphoglycerate kinase 1 |