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Peptidomic analysis reveals multiple protection of human breast milk on infants during different stages |
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| Authors: | Yahui Zhou Le Zhang Zhangbin Yu Aiqing Zhang Weimin Wu Wenjuan Chen Xiangyun Yan Heng Liu Yin Hu Chengyao Jiang Yan Xu Xingyun Wang Shuping Han |
| Affiliation: | 1. Department of Pediatrics, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China;2. Department of Pediatrics, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China Institute of pediatrics, Fourth Clinical Medicine College, Nanjing Medical University, Nanjing, China Department of Neonatology, Wuxi Children's Hospital affiliated to Nanjing Medical University, Wuxi, China Zhou, Zhang, Yu, and Zhang have contributed equally to this work.;3. Department of Pediatrics, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China Zhou, Zhang, Yu, and Zhang have contributed equally to this work.;4. Department of Pediatric Nephrology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China;5. Department of Pediatrics, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China Institute of pediatrics, Fourth Clinical Medicine College, Nanjing Medical University, Nanjing, China Department of Pediatrics, Fourth Clinical Medicine College, Nanjing Medical University, Nanjing, China;6. Department of Pediatrics, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China Department of Pediatrics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China |
| Abstract: | It has been shown that human breast milk (HBM) is an important nutrient for the growth and development of newborns. Currently, peptide drugs provide promising regimes in neonatal disease treatment, especially peptides from HBM that exhibit multiple functions within cells. To explore the potential biological function peptides among the colostrum, transition and mature milk from mother of extremely low birth weight children (the samples were collected from Women's Hospital of Nanjing Medical University from December 2016 to February 2017). A total of 3,182 nonredundant peptides were identified and compared among colostrum, transitional and mature milk using liquid chromatography/mass spectrometry technology, and the numbers and fragments of peptides were various. The isoelectric point and molecular weight analysis of the differentially expressed peptides basically accord with the range of mass spectrometry identification (<3 kDa). Gene Ontology analysis and Pathway analysis, restriction sites analysis, as well as bioinformatics analysis showed that these differentially expressed peptides enriched a variety of biological processes. We identified several putative peptides that might have bioactive effects in diseases and development of newborns, which will inform further functional investigations. Our preliminary research provided a better understanding of the function of peptides during the newborn periods. Furthermore, it laid a foundation for discovering new peptide drugs in neonatal disease treatment. |
| Keywords: | bioactive peptides colostrum mature milk transitional milk |