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An allogenic therapeutic strategy for canine spinal cord injury using mesenchymal stem cells |
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| Authors: | Irfan A Bhat Sivanarayanan T B Anjali Somal Sriti Pandey Mukesh K Bharti Bibhudatta S K Panda Indu B Megha Verma Anand J Arvind Sonwane G Sai Kumar Amarpal Vikash Chandra G Taru Sharma |
| Institution: | 1. Division of Physiology and Climatology, ICAR-Indian Veter 2. inary Research Institute, Izatnagar, Uttar Pradesh, India
Bhat and T. B. have contributed equally to this study.;3. Division of Veterinary Surgery, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India Bhat and T. B. have contributed equally to this study.;4. Department of Veterinary Physiology and Biochemistry, CSK HPKV Palampur (H.P.);5. inary Research Institute, Izatnagar, Uttar Pradesh, India;6. Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India;7. Division of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India;8. Division of Veterinary Surgery, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India |
| Abstract: | This study was conducted to characterize canine bone marrow-derived mesenchymal stem cells (BMSCs); in vivo tracking in mice, and therapeutic evaluation in canine clinical paraplegia cases. Canine BMSCs were isolated, cultured, and characterized in vitro as per International Society for Cellular Therapy criteria, and successfully differentiated to chondrogenic, osteogenic, and adipogenic lineages. To demonstrate the homing property, the pGL4.51 vector that contained luciferase reporter gene was used to transfect BMSCs. Successfully transfected cells were injected around the skin wound in mice and in vivo imaging was done at 6, 12 and 24 hr post MSCs delivery. In vivo imaging revealed that transfected BMSCs migrated and concentrated predominantly toward the center of the wound. BMSCs were further evaluated for allogenic therapeutic potential in 44 clinical cases of spinal cord injuries (SCI) and compared with conventional therapy (control). Therapeutic potential as evaluated by different body reflexes and recovery score depicted significantly better results in stem cell-treated group compared to control group. In conclusion, allogenic canine BMSCs can serve as potent therapeutic candidate in cell-based therapies, especially for diseases like SCI, where the conventional medication is not so promising. |
| Keywords: | allogenic canine characterization mesenchymal stem cells spinal injury |