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Neural stem cell-derived factors inhibit the growth and invasion of U87 stem-like cells in vitro |
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| Authors: | Xingxing Li Ruolan Tan Xiaoxuan Hu Qian Jiao Md Saidur Rahman Xinlin Chen Pengbo Zhang Jing An Haixia Lu Yong Liu |
| Institution: | 1. Institute of Neurobiology, School of Basic Medical Sciences and Key Laboratory of Environment and Genes Related to Diseases (Xi’an Jiaotong University), Ministry of Education of China, Xi’an Jiaotong University Health Science Center, Xi’an, China;2. Institute of Neurobiology, School of Basic Medical Sciences and Key Laboratory of Environment and Genes Related to Diseases (Xi’an Jiaotong University), Ministry of Education of China, Xi’an Jiaotong University Health Science Center, Xi’an, China
Department of Human Anatomy and Histoembryology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an, China;3. Department of Physiology, Medical College of Qingdao University, Qingdao, China;4. Department of Anesthesiology, The Second Affiliated Hospital, Xi’an Jiaotong University Health Science Center, Xi’an, China |
| Abstract: | Glioma is one of the most common and aggressive tumors in the brain. Significant attention has been paid to the potential use of neural stem/progenitor cells (NSCs/NPCs) as delivery vehicles to cure gliomas. However, whether the NSCs/NPCs or the factors they produced could make a contribution still remains to be seen. In this study, we focused on the inhibitory effects of the factors produced by NSCs/NPCs on the biological behavior of the glioma stem-like cell in vitro. The human glioma cell line U87 was selected and the U87 stem-like cells were addressed. After being cultured in the NSC condition medium (NSC-CM), the viability and proliferation of U87 stem-like cells were significantly reduced. The invasion of U87 stem-like cells and the migration of U87 cells were also significantly decreased. However, no significant change was observed in regard to the astrocytic differentiation of U87 stem-like cells. These indicated that NSCs/NPCs produced some factors and had an inhibitory effect on the growth and invasion but not the terminal differentiation of U87 stem-like cells. It is worth paying attention to NSCs/NPCs as a high-potential candidate for glioma treatment. |
| Keywords: | condition medium invasion neural stem/progenitor cells survival U87 stem-like cell |