microRNA-193b protects against myocardial ischemia-reperfusion injury in mouse by targeting mastermind-like 1 |
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Authors: | Jinzhu Zhang Jingjing Niu Baoqing Tian Meng Zhao |
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Affiliation: | Department of Cardiovascular Medicine, Linyi Central Hospital, Linyi, Shandong, China |
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Abstract: | The current study aimed to explore the functions and roles of microRNA-193b (miR-193b) in the myocardium with ischemia-reperfusion (I/R) injury and a potential therapeutic method for myocardial I/R injury. The mice were subjected to myocardial I/R with or without miR-193b pretreatment. The infarct size and myocardial enzymes were detected. The terminal deoxynucleotidyl transferase dUTP nick-end labeling assay was conducted to investigate the effect of miR-193b on cardiomyocyte apoptosis. The expression levels of miR-193b and mastermind-like 1 (MAML1) were validated by quantitative real-time polymerase chain reaction and Western blot analysis. The results suggested that the miR-193b expression level was significantly downregulated in the myocardium with I/R injury compared with control group. miR-193b overexpression is able to reduce infarct size and myocardial enzymes after myocardial I/R injury. Furthermore, overexpression of miR-193b could alleviate the apoptosis level after myocardial I/R injury. Taken together, the present study demonstrated that upregulated miRNA-193b alleviated myocardial I/R injury via targeting MAML1. |
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Keywords: | ischemic heart disease mastermind-like 1 microRNA-193b myocardial enzymes myocardial ischemia-reperfusion injury |
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