MicroRNA-328 ameliorates oxidized low-density lipoprotein-induced endothelial cells injury through targeting HMGB1 in atherosclerosis |
| |
Authors: | Chun-Yang Wu Zhao-Feng Zhou Bin Wang Zun-Ping Ke Zhong-Chun Ge Xian-Jin Zhang |
| |
Institution: | 1. Department of Cardiology, Yancheng Hospital Affiliated to Southeast University School of Medicine, Yancheng, China
Chun-Yang Wu, Zhao-Feng Zhou, and Bin Wang are co-first authors.;2. Department of Cardiology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China;3. Department of Cardiology, People's Hospital of Xuyi, Xuyi, China;4. Department of Intensive Care Unit, Huai'an Second People's Hospital and The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China |
| |
Abstract: | Atherosclerosis has been recognized as a chronic inflammatory disease, which can harden the vessel wall and narrow the arteries. MicroRNAs exhibit crucial roles in various diseases including atherosclerosis. However, so far, the role of miR-328 in atherosclerosis remains barely explored. Therefore, our study concentrated on the potential role of miR-328 in vascular endothelial cell injury during atherosclerosis. In our current study, we observed that oxidized low-density lipoprotein (ox-LDL)-induced human umbilical vein endothelial cells (HUVECs) apoptosis and inhibited cell viability dose-dependently and time-dependently. In addition, indicated dosage of ox-LDL obviously triggered HUVECs inflammation and oxidative stress process. Then, it was found that miR-328 in HUVECs was reduced by ox-LDL. HUVECs apoptosis was greatly repressed and cell survival was significantly upregulated by overexpression of miR-328. Furthermore, mimics of miR-328 rescued cell inflammation and oxidative stress process induced by ox-LDL. Oppositely, inhibitors of miR-328 strongly promoted ox-LDL-induced endothelial cells injury in HUVECs. By using bioinformatics analysis, high-mobility group box-1 (HMGB1) was predicted as a downstream target of miR-328. HMGB1 has been reported to be involved in atherosclerosis development. The correlation between miR-328 and HMGB1 was validated in our current study. Taken these together, it was implied that miR-328 ameliorated ox-LDL-induced endothelial cells injury through targeting HMGB1 in atherosclerosis. |
| |
Keywords: | atherosclerosis high-mobility group box-1 microRNA-328 oxidized low-density lipoprotein |
|
|