In vitro and histological investigation of antitumor effect of some triazole compounds in colon cancer cell line |
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| Authors: | Akif Evren Parlak Suat Tekin Arzu Karatepe Pelin Koparir Hafize Telceken Ali Osman Ceribası Mustafa Karatepe |
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| Affiliation: | 1. Department of Environmental Protection Technologies, Programme of Environmental Protection and Control, Keban Vocational School of Higher Education, Firat University, Elazig, Turkey;2. Department of Physiology, Faculty of Medicine, Inonu University, Malatya, Turkey;3. Department of Chemistry, Faculty of Science and Arts, Bingol University, Bingol, Turkey;4. Department of Chemistry, Forensic Medicine Institute, Malatya, Turkey;5. Department of Chemistry, Faculty of Science, Firat University, Elazig, Turkey;6. Department of Pathology, Faculty of Veterinary, Firat University, Elazig, Turkey |
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| Abstract: | 1,2,4-Triazoles are used as antifungal, antibacterial, antimicrobial, and antioxidant against some oxidative radical species. Recently, many 1,2,4-triazoles continue to be synthesized. In this study, the effect of the 1,2,4-triazole derivatives on human colon cancer (HT29) was investigated in vitro and in vivo in rats. MTT test was applied to in in vitro experiments. For in vivo study, rats were divided into seven groups as follows: Control group (negative control), azoxymethane (AOM), AOM + cisplatin 15, AOM + L1, AOM + L2, AOM + L3, and AOM + L4. To create colon cancer, the AOM injection was injected subcutaneously at a dose of 15 mg/kg, three times (once weekly). The in vivo studies were completed at 28 weeks. It was found that the 1,2,4-triazole derivatives reduced the cell viability (P < 0.05). In all animals in the experimental groups, mild dysplasia was detected in 100% of the colon mucosal epithelium. Severe dysplasia and adenocarcinoma were observed in L1 groups. As a result, this study determined that the 1,2,4-triazole derivatives exhibit antitumor activity. |
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| Keywords: | azoxymethane colon cancer HT29 1,2,4-triazole |
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