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Electrospun poly-l-lactic acid/polyvinyl alcohol nanofibers improved insulin-producing cell differentiation potential of human adipose-derived mesenchymal stem cells
Authors:Mohammad Ojaghi  Fatemeh Soleimanifar  Alireza Kazemi  Marzieh Ghollasi  Masoud Soleimani  Nikoo Nasoohi  Seyed Ehsan Enderami
Affiliation:1. Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran;2. Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran;3. Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran;4. Department of Cell and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran;5. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;6. Stem Cell Technology Research Center, Tehran, Iran
Abstract:Combination of adipose-derived mesenchymal stem cells (ADSCs) and synthetic materials in terms of pancreatic tissue engineering can be considered as a treatment of diabetes. This study aimed to evaluate the differentiation of human ADSCs to pancreatic cells on poly-l -lactic acid/polyvinyl alcohol (PLLA/PVA) nanofibers as a three-dimensional (3D) scaffold. Mesenchymal stem cells (MSCs) were characterized for mesenchymal surface markers by flow cytometry. Then ADSCs were seeded on 3D scaffolds and treated with pancreatic differentiation medium. Immunostaining assay showed that ADSCs were very efficiently differentiated into a relatively homogeneous population of insulin-producing cells. Moreover, real-time RT-PCR results revealed that pancreas-specific markers were highly expressed in 3D scaffolds compared with their expression in tissue culture plates and this difference in expression level was significant. In addition, insulin and C-peptide secreted in response to varying concentrations of glucose in the 3D scaffold group was significantly higher than that in 2D culture. The results of the present study confirmed that PLLA/PVA scaffold seeded with ADSCs could be a suitable option in pancreatic tissue engineering.
Keywords:adipose-derived mesenchymal stem cells (ADSCs)  differentiation  insulin-producing cells  pancreatic tissue engineering  poly-l-lactic acid/polyvinyl alcohol (PLLA/PVA)  scaffold
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